pSiteExplorer: Visualization of the mass spectrometric data underlying phospho-site quantification

Abstract

Mass spectrometry based phospho-proteomics is a widely used approach to assess protein phosphorylation. Intensities of phospho-peptide ions are obtained by integrating the MS signal over their chromatographic peaks. How individual peptide measurements mapping to the same phospho-site are combined for the quantification of the given site is, however, in most cases hidden from researchers conducting, reviewing, and reading these studies. I here describe pSiteExplorer, an R script that visualizes the peak intensities associated with phospho-sites in MaxQuant output tables. Barplots of MS intensities originating from phospho-peptides with distinct amino acid sequences due to missed cleavages, different numbers of phosphates and from all off-line chromatographic fractions and charge states are displayed. This tool will help gaining a deeper insight into phospho-site quantifications by contrasting individual and summed phospho-peptide intensities with the site-level values derived by MaxQuant. This will support the validation of quantification results, for example, for the selection of candidates for follow-up studies.