Denys Oliinyk

This study uses Aggregate Deep Visual Proteomics to show that α-synuclein inclusions selectively concentrate specific proteins in neurons, revealing their formation mechanisms and potential therapeutic targets.

The paper introduces the ZenoTOF 8600 system, which enhances proteomics by increasing ion flux and improving precursor identification and quantification in DIA mode.

The paper introduces pSPEC, a reproducible proteomic sample preparation method for biofluids that increases protein identification by ~20% compared to in-solution plasma.

The paper constructs a cell type-resolved proteomic atlas of the human body using Deep Visual Proteomics, revealing protein abundance patterns and uncovering cancer-testis antigens in oocytes.

This study develops a de novo insulin receptor agonist, RF-409, which effectively treats severe insulin resistance in a mouse model by activating a mutant receptor and improving glucose homeostasis.

The paper introduces SPEC, a proteomics sample preparation method that improves proteome depth and reproducibility from limited samples.

nanoPhos improves phosphoproteomics sensitivity and enables cell-type resolved spatial analysis of mouse brain tissue.

This study evaluates diaPASEF for high-sensitivity identification and quantification of HLA-I peptides, demonstrating improved detection of neoantigens in cancer models.

EXCRETE improves exoproteome identification in cyanobacteria, revealing 85% of predicted secreted proteins and highlighting key functions in cell envelope maintenance and nutrient acquisition.

The paper investigates how 22 post-translational modifications affect peptide collision cross sections using ion mobility spectrometry, finding sequence- and modification-specific effects.

μPhos is a phosphoproteomics platform that enables the identification of 5,000 phosphorylation sites from 5 µg of sample, supporting extensive perturbation experiments.

This study shows that astrocyte-derived cholesterol, released via apoE, promotes neuroinflammation by enhancing inflammatory signaling in immune cells.

This study demonstrates the application of dia-PASEF with TIMS to achieve high-throughput phosphoproteomics with short gradients, quantifying over 12,000 phosphopeptides in one hour.