Expression of β-catenin, LEF-1, MMP7 and SFRP5 associated with poor outcome in pancreatic cancer

Abstract

Pancreatic cancer is highly aggressive and lethal malignancies worldwide. {beta}-catenin, LEF-1, MMP7 and SFRP5 are key regulatory proteins in the Wnt signaling pathway. It has been confirmed that Wnt signaling pathway is involved in the biological regulation of pancreatic cancer cells and plays an important role. However, its effect on the malignant phenotypes of pancreatic cancer cell and the corresponding molecular details remains unknown. In the current study, the expression of {beta}-catenin, LEF-1 and MMP7 was up-regulated and the expression of SFRP5 was down-regulated in pancreatic cancer cell lines and tissues by RNA sequencing, quantitative PCR (qPCR) and immunohistochemical analysis. {beta}-catenin, LEF-1 and MMP7 positive expression correlates with poor prognosis in PDAC patients, and SFRP5 positive expression correlates with longer survival times. Subsequently r software was used to establish a rosette model to predict the prognosis of pancreatic cancer according to the results of multi-factor analysis. After statistical analysis, age, CA-199, {beta}-catenin, MMP7 and SFRP5 could serve as promising biomarkers for predicting prognosis in PDAC patients. This study also suggests that survival probability of patients can be reliably predicted by a nomogram-based method.