bioRxivBiophysicsPreprint
Super-resolution imaging reveals dynamic reticular cytoophidia
Abstract
CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells from all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using EGFP dimeric tag, as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components such as IMPDH. In addition, we observe CTPS granules with tentacles.
- organism: human
- assay: fluorescence / live-cell microscopy
- research focus: cell type annotation
- approach: microscopy & imaging analysis
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