J.-L. Liu

Filamentation and inhibition of prokaryotic CTP synthase

This study determines the structure of E. coli CTPS filament and identifies regulatory mechanisms, revealing a solvent-accessible ammonia tunnel and distinct CTP binding mode.

Cytoophidia influence cell cycle and size in Schizosaccharomyces pombe

This study shows that cytoophidia of CTPS regulate cell cycle and size in S. pombe by affecting G2/M transition and cell growth genes.

Dynamic atP5CS2 Filament Facilitates Substrate Channeling

This study identifies a filament structure in Arabidopsis thaliana P5CS2 that facilitates substrate channeling, enhancing catalytic efficiency in proline synthesis under abiotic stress.

Structural basis for activation of glutaminase

This paper determines the structure of human glutaminase with phosphate, revealing an allosteric activation mechanism and showing phosphate antagonizes known inhibitors.

Structural basis of bifunctional CTP/dCTP synthase

This study determines the structure of CTP/dCTP synthase bound to deoxynucleotides, revealing its bifunctional mechanism and cellular compartmentation potential.

GTP cycling gates CTP synthase activation

This study reveals how GTP cycling gates CTP synthase activation through three sequential checkpoints, linking GTP binding and dissociation to catalytic steps in CTP synthesis.

Super-resolution imaging reveals dynamic reticular cytoophidia

The paper uses super-resolution microscopy to show that CTPS forms dynamic, reticular structures called cytoophidia in human cells, which may accommodate other enzymes like IMPDH.

Structural basis of human PRPS2 filaments

This study determines the structure of hPRPS2 polymers using cryo-EM, revealing how ADP binding affects polymerization and catalytic activity.

Fat body-specific reduction of CTPS alleviates HFD-induced obesity

This study shows that reducing CTPS in the fat body of Drosophila alleviates HFD-induced obesity by decreasing lipid storage and TAG levels.

Allosteric inhibition of PRPS is moderated by filamentous polymerization

The paper investigates two filament structures of E. coli PRPS resolved by Cryo-EM, revealing how ligand binding modulates allosteric inhibition and PRPP production.

Molecular Crowding Facilitates Bundling of IMPDH Polymers and Cytoophidium Formation

This study shows that molecular crowding promotes IMPDH polymer bundling and cytoophidium formation, with implications for protein stability and subcellular compartment function.

Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe

This study investigates how ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe by identifying ubiquitination regulators and deubiquitinating enzymes involved in CTPS filamentation.

Structural basis of dynamic P5CS filaments

This study determines the structure of Drosophila P5CS filament, revealing how filamentation coordinates GK and GPR domains for catalysis.

CTP synthase forms cytoophidia in archaea

This study shows that CTP synthase forms cytoophidia in archaea, extending the known presence of these structures to all three domains of life.

The proline synthesis enzyme P5CS forms cytoophidia in Drosophila

The paper identifies P5CS as a CTPS-interacting protein in Drosophila and shows they form cytoophidia, linking two enzymes in pyrimidine and proline biosynthesis.

TurboID-mediated proximity labelling of cytoophidium proteome in Drosophila

The paper applies TurboID-mediated biotinylation to characterize the cytoophidium proteome in Drosophila, demonstrating its utility for studying subcellular compartments.