CRISPR-Based Genome Editing in Harmonia Axyridis

Abstract

Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. Previous studies have employed double-stranded RNA techniques to investigate the functional genomics of this species. In this study, we utilized the CRISPR-Cas9 approach to achieve precise genome editing in H. axyridis. Specifically, we targeted two distinct genes known to exhibit visible phenotypic effects. While the knockout of the laccase2 gene resulted in an early-detectable phenotype but also in lethality, we successfully established a viable and genetically stable mutant colony by disrupting the scarlet gene. Our findings contribute to the expanding knowledge of genetic manipulation in H. axyridis and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.