Plug-and-Play 3D localization microscopy
Ori Refael CoheniD, Dafei XiaoiD, Reut Orange KedemiD, Onit AlaloufiD, Prakash JoshiiD, Y. NakataniiD, Anna‐Karin GustavssoniD, Yoav ShechtmaniD
Abstract
Point-spread-function engineering by depth-encoding phase masks enables volumetric super-resolution imaging by 3D single-molecule localization microscopy (SMLM) but usually requires cumbersome relay optics. We demonstrate simple 3D SMLM implementation by phase mask insertion directly into the infinity space of a commercial microscope, along with appropriate computational compensation for field-dependence. The entire inserted component, including the phase mask, is 3D printing-based.
02 /Specification & Classification
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