Advancing Inter-brain Synchrony Measurement: A Comparative Hyperscanning Study of High-Density Diffuse Optical Tomography and Functional Near-infrared Spectroscopy

Abstract

Inter-brain synchrony (IBS), measured by hyperscanning, refers to the synchronization of multiple individuals brain activities during social interactions. Traditional fNIRS-based hyperscanning suffers shortcomings like low spatial resolution and high susceptibility to superficial interference, causing imprecise estimation of IBS in complex social tasks. This study aims to fill the knowledge gap by comprehensively assessing how high-density diffuse optical tomography (HD-DOT), an enhanced alternative to fNIRS, can benefit hyperscanning studies of complex social interactions. Sixteen dyads were engaged in both collaborative and individual tangram puzzle tasks, and their brain activities were recorded simultaneously using HD-DOT and fNIRS. We found that HD-DOT demonstrated significantly stronger IBS and identified more brain regions with significant IBS compared to fNIRS during the collaborative task. Specifically, while fNIRS detected IBS only in the dorsolateral prefrontal cortex (DLPFC) and supramarginal gyrus (SMG), HD-DOT revealed additional IBS in the superior temporal gyrus (STG). Additionally, compared to the individual task, the collaborative task showed increased IBS in HD-DOT, not only in the DLPFC but also in the SMG, frontal eye fields (FEF), and inferior frontal gyrus (IFG). By highlighting the superior spatial resolution and sensitivity of HD-DOT in capturing detailed and extensive neural activity during complex social interactions, our findings for the first time clarified the potential strengths of HD-DOT in measuring IBS over traditional fNIRS. These advances provide a stronger empirical foundation for investigating the neural basis of social interaction, paving the way for future research on real-world, dynamic group behaviors. HighlightsO_LIFirst application of HD-DOT for inter-brain synchrony measurement. C_LIO_LIFirst demonstration of HD-DOTs superiority over fNIRS in IBS measurement. C_LIO_LIHD-DOT detects stronger IBS and more brain regions than fNIRS. C_LI