Laboratory and field trials reveal the potential of a gel formulation of entomopathogenic nematodes as biocontrol against the fall armyworm (Spodoptera frugiperda)

Abstract

1.The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae) can cause tremendous yield losses in maize. Its invasion into Africa and Asia has led to an enormous influx of insecticides into maize agro-ecosystems. Safe, effective and readily available alternatives are desperately needed. Entomopathogenic nematodes (EPN) are commonly used against soil insect pests, but can also control some above-ground pests. We explored the possibility to control FAW by incorporating EPN into a formulation that can be easily applied into the whorl of maize plants, where the caterpillars mostly feed. We tested this approach in laboratory cage experiments as well as in field trials. In the laboratory, treating maize plants with a low dose of EPN in a carboxymethyl cellulose (CMC) gel formulation (about 3000 infective juveniles per plant), caused 100% mortality of FAW caterpillars and prevented plant damage considerably, whereas EPN applied in water or a surfactant-polymer-formulation (SPF) caused 72% and 94% mortality, respectively. Under field conditions, one-time treatments with EPN applied in water, SPF or CMC gel were all able to prevent significant plant damage, but only the EPN-gel formulation significantly reduced FAW infestation. Notably, the gel formulation was as effective as a standard dose of cypermethrin, an insecticide commonly used against FAW. Repeated applications may be needed to reduce re-infestations by FAW across a whole cropping season depending on the local maize phenology and pest dynamics. These findings demonstrate that EPN are excellent candidates for the biological control of FAW and are a safe and sustainable alternative to chemical insecticides. HighlightsO_LIEntomopathogenic nematodes are highly lethal to fall armyworm caterpillars. C_LIO_LIAppropriate formulation of the nematodes is crucial for above-ground application. C_LIO_LIA gel formulation of entomopathogenic nematodes was as effective as chemical insecticides. C_LIO_LIEntomopathogenic nematodes can be used for the control of fall armyworm in maize. C_LI