Identification of the conserved iol gene cluster involved in rhizosphere competence in Pseudomonas

Abstract

The Pseudomonas genus has shown great potential as a sustainable solution to support agriculture through its plant-growth promoting and biocontrol activities. However, their efficacy as bioinoculants is limited by unpredictable colonization in natural conditions. Our study identifies the iol locus, a gene cluster in Pseudomonas involved in inositol catabolism, as a feature enriched among superior root colonizers in natural soil. Further characterization revealed that the iol locus increases competitiveness by inducing swimming motility and fluorescent siderophore production in response to inositol, a plant-derived compound. Public data analyses indicate that the iol locus is broadly conserved in the Pseudomonas genus and linked to diverse host-microbe interactions. Our findings suggest the iol locus as a potential target for developing more effective bioinoculants, given its conservation and association with diverse host-microbe interactions.