bioRxivBioengineeringPreprint
Straightforward semi-quantitative MALDI-TOF MS based screening approach for selection of recombinant protein producing E. Coli
I. N. Kravtsov, A. I. Solovyev, E. A. Potemkina, A. V. Kartashova, M. A. Dmitrieva, K. V. Danilova, I. L. Tutykhina, N. B. Polyakov, D. A. Egorova
Abstract
MALDI-TOF MS represents a rapid and cost-effective method for identifying proteins and microorganisms. When obtaining recombinant protein producers, differences in expression levels among transformants necessitate the conduction of a small-scale screening of expression levels. This study proposes a fast and easy method for screening clones producing recombinant proteins using MALDI-TOF MS. Various recombinant proteins were utilized to test the proposed method
- organism: E. coli
- assay: proteomics
- research focus: protein folding & dynamics
- approach: experimental assay
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