Adjuvants to improve efficacy of miticides in managed honey bee (Apis mellifera) colonies to control Varroa destructor

Abstract

Beekeepers must manage Varroa destructor mites to maintain colony health. Large-scale beekeepers often use chemical treatments (miticides) to manage this pest. Miticide resistance drives a need for compounds with alternative modes of toxic action that can be used in a rotation as part of a Varroa management plan. This research aimed to determine the efficacy of oxalic acid, clove oil, and fenpyroximate when delivered in glycerin soaked in strips and combined with a range of bee-safe adjuvants. Adjuvants are a group of compounds used in plant pesticide applications to increase the spreading and penetration of a pesticide. Laboratory cage trials tested a miticidal active ingredient (oxalic acid, clove oil, or fenpyroximate) and an adjuvant (Ecostep BC-12(R), Ecostep SE-11(R), Ecostep AE-13(R), Ecostep CE-13(R), or Silwet L-7500(R)) in glycerin-soaked strips; field trials evaluated oxalic acid combined with Ecostep BC-12(R) adjuvant in glycerin-soaked strips. Field trials with oxalic acid and adjuvant in glycerin caused a significant decrease in Varroa in year 1 and a significant reduction in Varroa, relative to the solvent control, in year 2. On the other hand, field trials with oxalic acid alone did not reduce Varroa loads in colonies relative to the solvent control. Additionally, mite drop data indicate increased speed for the miticidal effect when an adjuvant is included with oxalic acid. This research informs formulation chemistries for oxalic acid and other miticides to help beekeepers maintain healthy hives.