Effect of different odors on the rat urine proteome

Do rats have corresponding changes in their urinary proteome when smelling different odors? In this study, urine samples were collected from six rats after smelling sesame oil and essential balm for three days. And samples were collected before and on the third and fourth days. Comparing the urinary protein groups of Day0 and Day4 of the sesame oil group, 143 differential proteins were identified, and the average number of randomly generated differential proteins was 7.3, which means that about 95% of the differential proteins could not be randomly generated. in the sesame oil group, differential proteins such as low-density lipoprotein receptor-related protein 2 and fetuin B, a biomarker of COPD, which are associated with olfaction, were identified. While uteroglobulin, trichothecene factor 3, and visfatin 2 were identified in the essential balm group, which had significant changes and were related to the production of olfactory sensation. It is noteworthy that we identified odor-binding protein 2A in the essential balm group, which was present in the e-cigarette model. This study demonstrates that odor can affect rat urinary proteome, with different odors affecting it differently. This provides a new approach to explore the biological process of olfaction.