Melanin pathway genes regulate color and morphology of butterfly wing scales

Abstract

The cuticular skeleton of a butterfly wing scale cell serves both as a substrate for the deposition of pigments and as an exquisitely finely-sculpted material responsible for the production of structural colors. While cuticle rigidity and pigmentation depend to a large extent on the end products of a branched biochemical pathway - the melanin pathway - little is known whether genes in this pathway also play a role in the development of specific scale morphologies that might aid in the development of structural colors. Here we first show that male and female Bicyclus anynana butterflies display differences in scale size and scale morphology but no differences in scale color. Then we use CRISPR/Cas9 to show that knockout mutations in five genes that function in the melanin pathway, TH, DDC, yellow, ebony, and aaNAT, affect both the fine structure and the coloration of the wing scales. Most dramatically, mutations in yellow led to extra horizontal cuticular laminae on the surface of scales, whereas mutations in DDC led to taller and sheet-like vertical cuticular laminae throughout each scale. We identify some of the first genes affecting the development of scale morphology, and whose regulation and pleiotropic effects may be important in creating, as well as limiting, the diversity of structural as well as pigmentary colors observed in butterflies.