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Endangered Przewalski's horse, Equus przewalskii, cloned from historically cryopreserved cells

B. J. Novak, O. A. Ryder, M. L. Houck, A. S. Putnam, K. Walker, L. Russell, B. Russell, S. Walker, S. S. Arenivas, L. Aston, G. Veneklasen, J. A. Ivy, K.-P. Koepfli, A. Rusnak, J. Simek, A. Zhuk, R. Phelan

PDFDOIPreprint

Abstract

Two endangered Przewalskis horse stallions were cloned from fibroblast cells cultured and cryopreserved in 1980. These stallions are clones of a male that lived from 1975-1998 that pedigree analyses identified as a genetically valuable male for present-day conservation breeding. This is the first time that multiple healthy clones have been produced for an endangered species.

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