Go west: population genomics reveals unexpected population fluctuations and little gene flow in Western Hemisphere populations of the predatory lady beetle, Hippodamia convergens

Abstract

The convergent lady beetle, Hippodamia convergens, is used extensively in augmentative biological control of aphids, thrips, and whiteflies across its native range in North America, and was introduced into South America in the 1950s. Overwintering H. convergens populations from its native western range in the United States are commercially collected and released across its current range in the eastern U.S., with little knowledge of the effectiveness of augmentative biological control using H. convergens. Here we use a novel ddRADseq-based SNP/haplotype discovery approach to estimate its range-wide population diversity, differentiation, and recent evolutionary history. Our results indicate (1) significant population differentiation among eastern U.S., western U.S., and South American populations of H. convergens, with (2) little to no detectable recent admixture between them, despite repeated population augmentation, and (3) continued recent population size expansion across its range. These results contradict previous findings using microsatellite markers. In light of these new findings, the implications for the effectiveness of augmentative biological control using H. convergens are discussed. Additionally, because quantifying the non-target effects of augmentative biological control is a difficult problem in migratory beetles, our results could serve as a foundation cornerstone in improving and predicting the efficacy of future releases of H. convergens across its range.