Conditioning dentinal collagen by glycation improves long-term bond strength

Abstract

ObjectivesThis study examined methylglyoxal (MGO) as a collagen crosslinker to reinforce demineralized dentin, enhance its enzymatic resistance, and improve long-term durability of the resin-dentin bond. MethodsDemineralized dentinal collagen films were treated with 0.5, 1, or 3 M MGO and subsequently exposed to collagenase to assess their resistance to enzymatic degradation. MGO-induced crosslink formation was monitored using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) spectroscopy by tracking the carbohydrate-associated band at 1180 cm-{superscript 1}. The apparent elastic modulus of the treated specimens was measured using a three-point bending test. For bond strength evaluation, resin-dentin beams were prepared and tested using microtensile bond strength (TBS) to assess the influence of MGO pretreatment on interfacial adhesion. ResultsThe ATR-FTIR spectra demonstrated increased intensity in the carbohydrate double bands (1000-1180 cm-{superscript 1}) in glycated samples compared to the control. Glycation with 3 M MGO exhibited the highest resistance to enzymatic degradation, persisting for up to 60 hours with a 78-fold increase in resistance factor compared to the control group (p View larger version (24K): org.highwire.dtl.DTLVardef@54ae32org.highwire.dtl.DTLVardef@1787fdforg.highwire.dtl.DTLVardef@130b40org.highwire.dtl.DTLVardef@47c5f9_HPS_FORMAT_FIGEXP M_FIG C_FIG