Beyond the Billion: Dose-Response Immunophenotyping and Machine Learning Classification of Live versus Heat-Inactivated Gram-Positive Probiotic Strains in Human Peripheral Blood Mononuclear Cells
Giovanni Deusebio, Annalisa Visciglia, Angela Amoruso, Marco Pane
This study challenges assumptions about probiotic efficacy by showing heat-treated strains can match live strains in immunological activity at higher doses, using flow cytometry and machine learning to classify probiotic strains.
Frontiers in Microbiology · Oct 26, 2026Peer-reviewedMicrobiology