Author

A. A. Lievano

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 1 Immunology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:human· 2 Assay:CRISPR screen· 1 Research Focus:gene regulatory network inference· 1 Research Focus:variant calling· 1 Approach:wet-lab / biochemical assay· 1

Frequent Co-Authors

J. A. Jarrell· 2 shared K. H. W. J. Lau· 2 shared R. S. Pawell· 2 shared A. A. Twite· 1 shared B. J. Sytsma· 1 shared C. Priest· 1 shared D. Gottlieb· 1 shared F. L. Pan· 1 shared

Activity by Year

2019-2020 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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2 shared papersImmunology

J. A. Jarrell

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2 papers

Recent·Most cited

Genome editing human primary T cells with microfluidic vortex shedding & CRISPR Cas9

J. A. Jarrell, B. J. Sytsma, L. H. Wilson, F. L. Pan, K. H. W. J. Lau, G. T. S. Kirby, A. A. Lievano, R. S. Pawell

This study evaluates microfluidic vortex shedding (VS) and electric field-enhanced VS (eVS) for delivering CRISPR-Cas9 RNP to primary human T cells, showing improved editing efficiency and cell viability compared to electroporation.

bioRxiv · Feb 26, 2020 · PreprintImmunology

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding

J. A. Jarrell, A. A. Twite, K. H. W. J. Lau, M. N. Kashani, A. A. Lievano, J. Acevedo, C. Priest, J. Nieva, D. Gottlieb, R. S. Pawell

This study develops a microfluidic vortex shedding method to deliver mRNA into human T cells, achieving high transfection efficiency and cell viability.

bioRxiv · Jan 5, 2019Bioengineering