Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase
A. A. Malaer, N. Wili, L. A. Voelker, M. I. Kozlova, R. Cadalbert, A. Daepp, M. E. Weber, J. Zehnder, G. Jeschke, H. Eckert, A. Bockmann, D. Klose, A. Y. Mulkidjanian, B. H. Meier, T. Wiegand
The paper captures a late transition state of ATP hydrolysis in a bacterial helicase using NMR spectroscopy and identifies key interactions involving aluminum fluoride.
bioRxiv · Apr 8, 2021Biophysics