High-yield Production of Recombinant Platelet Factor 4 Protein by Harnessing and Honing the Gram-negative Bacterial Secretory Apparatus
S. Ataei, M. N. Taheri, F. Taheri, F. Zare, N. Amirian, A. Behzad-Behbahani, A. Rahimi, G. Tamaddon
This study develops a method to produce recombinant platelet factor 4 (PF4) using the type II secretory pathway in E. coli, achieving high yields and structural fidelity.
bioRxiv · Nov 4, 2019Bioengineering