A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus
D. Conde, P. M. Triozzi, K. M. Balmant, A. L. Doty, M. Miranda, A. Boullosa, H. W. Schmidt, W. J. Pereira, C. Dervinis, M. Kirst
The paper presents a method to isolate nuclei from plant tissues for scRNA-seq, overcoming limitations of protoplast isolation in complex tissues.
bioRxiv · Feb 11, 2021Plant Biology