M. Kirst

Mesophyll-Specific Circadian Dynamics of CAM Induction in the Ice Plant Unveiled by Single-Cell Transcriptomics

The paper uses snRNA-seq to study CAM induction in ice plant mesophyll cells, revealing circadian gene expression differences between CAM and C3 pathways.

The Single-Cell Transcriptome Program of Nodule Development Cellular Lineages in Medicago truncatula

The paper characterizes single-cell transcriptomes during Medicago truncatula nodule development, revealing dynamic changes in auxin and cytokinin signaling pathways across cell lineages.

The western redcedar genome reveals low genetic diversity in a self-compatible conifer

The paper assembles the western redcedar genome and finds it has low genetic diversity, suggesting mechanisms like associative overdominance maintain genetic variation despite selfing.

Two shifts in evolutionary lability underlie independent gains and losses of root-nodule symbiosis in a single clade of plants

The paper reconstructs the evolutionary history of root-nodule symbiosis in the nitrogen-fixing clade, identifying a two-step process involving shifts in evolutionary lability that explain multiple independent gains and losses of the trait.

Single-cell analysis resolves the genetic program of primary vascular system formation in hybrid poplar

The paper uses single-cell RNA-seq to map the genetic program of primary vascular development in hybrid poplar at single-cell resolution.

Temporal change in chromatin accessibility predicts regulators of nodulation in Medicago truncatula

The paper identifies regulators of legume-rhizobia symbiosis by analyzing temporal chromatin accessibility and gene expression in Medicago truncatula roots.

Functional and comparative genomics reveals conserved noncoding sequences in the nitrogen-fixing clade

The paper identifies conserved noncoding sequences in nitrogen-fixing plants and shows their role in regulating genes involved in root nodule symbiosis.

Spatiotemporal cytokinin signaling imaging reveals IPT3 function in nodule development in Medicago truncatula

A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus

The paper presents a method to isolate nuclei from plant tissues for scRNA-seq, overcoming limitations of protoplast isolation in complex tissues.

Molecular changes in Mesembryanthemum crystallinum guard cells underlying the C3 to CAM transition

This study identifies transcriptomic changes in guard cells of M. crystallinum during salt-induced C3 to CAM transition, showing guard cells can undergo this shift.

Cross-talk between active DNA demethylation, resetting of cellular metabolism and shoot apical growth in poplar bud break

The paper shows that DNA demethylation, triggered by chilling, regulates the transition from winter dormancy to shoot growth in poplar by affecting gene expression and metabolism.

A DNA demethylase overexpression promotes early apical bud maturation in poplar through the biosynthesis and accumulation of flavonoids

This study shows that overexpressing a DNA demethylase gene accelerates apical bud maturation in poplar by promoting flavonoid biosynthesis under short-day and cold conditions.