Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a
C. Patinios, S. T. de Vries, M. Diallo, L. Lanza, P. L. J. V. Q. Verbrugge, A. M. Lopez-Contreras, J. van der Oost, R. A. Weusthuis, S. W. M. Kengen
This paper develops a CRISPR-Cas12a system for efficient genome engineering in Clostridium beijerinckii NCIMB 8052, achieving single-gene knockouts and multiplex editing.
bioRxiv · Aug 22, 2022 · PreprintMicrobiology