Near-Physiological in vitro Assembly of 50S Ribosomes Involves Parallel Pathways
X. Dong, L. K. Doerfel, K. Sheng, J. N. Rabuck-Gibbons, A. M. Popova, D. Lyumkis, J. R. Williamson
The paper reports the assembly of 50S ribosome intermediates using in vitro reconstitution and cryo-EM, revealing parallel assembly pathways and cooperative blocks.
bioRxiv · Nov 9, 2022Biochemistry