Precise exogenous insertion and sequence replacements in poplar by simultaneous HDR overexpression and NHEJ suppression using CRISPR-Cas9
A. Movahedi, H. Wei, X. Zhou, J. C. Fountain, Z.-H. Chen, Z. Mu, W. Sun, J. Zhang, D. Li, B. Guo, R. K. Varshney, L. Yang, Q. Zhuge
This paper improves HDR efficiency for gene knock-in in poplar by combining XRCC4 silencing with CtIP and MRE11 overexpression, achieving higher knock-in rates and fewer InDels.
bioRxiv · Jul 4, 2020Plant Biology