Nitrogenase resurrection and the evolution of a singular enzymatic mechanism
A. K. Garcia, D. Harris, A. Rivier, B. Carruthers, A. P. Barros, L. Seefeldt, B. Kacar
This study resurrects ancient nitrogenase enzymes and shows their in vivo functionality and reversible dinitrogen reduction mechanism, suggesting evolutionary constraints in catalyzing this reaction.
eLife · Feb 17, 2023Peer-reviewedEvolutionary Biology