Highly sensitive and full-genome interrogation of SARS-CoV-2 using multiplexed PCR enrichment followed by next-generation sequencing
Chenyu Li, David N. Debruyne, Julia Spencer, Vidushi Kapoor, Lily Y. Liu, Bo Zhou, Utsav Pandey, Moiz Bootwalla, Dejerianne G. Ostrow, Dennis T. Maglinte, David Ruble, Alex Ryutov, Li Shen, Lucie Lee, Rounak Feigelman, , Zhitong Liu
A 343-primer-pair multiplex PCR assay enables electrophoretic detection and full-genome sequencing of SARS-CoV-2 across 8 to half a million viral copies from 13 clinical specimens.
bioRxiv · Mar 12, 2020 · PreprintGenomics