Screening and structural characterization of a nanobody targeting the thermostable green fluorescent protein
Yaning Li, Weijie Gu, Aimin Ni, Dianfan Li, Tingting Li
The paper identifies and characterizes a nanobody, Sb32, that binds thermostable GFP (TGP) with nanomolar affinity and reveals an unusual binding mechanism involving CDR2 and hydration networks.
Acta Biochimica et Biophysica Sinica · Dec 31, 2025Peer-reviewedBiophysics