A novel cell indirect calorimetry method unveils the metabolic fluxomic signatures of human monocyte derived M(LPS+INF-γ) and M(IL-4) macrophages
Gloria Cinquegrani, Valentina Spigoni, Federica Fantuzzi, Anna D’Antuono, Francesca Bagnaresi, Elisabetta Giordano, Alberto Burato, Alessandra Dei, Riccardo C. Bonadonna
This study develops a novel indirect calorimetry method to quantify metabolic fluxes in human macrophages and reveals distinct metabolic signatures between M(LPS+INF-γ) and M(IL-4) subsets.
bioRxiv · Oct 21, 2025 · PreprintPhysiology