Thermodynamics shape the in vivo enzyme burden of glycolytic pathways
Daven B. Khana, Annie Jen, Evgenia Shishkova, Kirsten Fisher, Eashant Thusoo, Jonathan Williams, Alex Henkel, David M. Stevenson, Joshua J. Coon, Daniel Amador‐Noguez
The paper quantifies enzyme burdens in glycolytic pathways of three bacteria, finding that thermodynamically favorable pathways require less enzymatic protein to sustain the same flux.
mBio · Sep 16, 2025Peer-reviewedSystems Biology