EnZight: A Structure-Guided Algorithm to Identify and Prioritize Substitution Hotspots for Enzyme Engineering
Rune Rahbek Oestergaard, Mikkel Lyskjær Jensen, Suzana Siebenhaar, Deniz Bicer, Peter Wad Sackett, Alexander Andersen, Matteo Tiberti, Elena Papaleo, Serina L. Robinson, Søren Thirup, Peter Westh, Laura Rotilio, Jens Preben Morth
EnZight identifies and prioritizes substitution hotspots in enzymes using structural alignment, demonstrating effectiveness in improving catalytic activity and thermostability.
bioRxiv · Sep 10, 2026 · PreprintBioengineering