Combinatorial mutagenesis of N-terminal sequences reveals unexpected and expanded stability determinants of the Escherichia coli N-degron pathway
Sabyasachi Sen, Nastassja Corrado, Alexander R. Tiso, Khai Khee Kho, Aditya M. Kunjapur
The paper uses combinatorial mutagenesis to identify stability determinants of the Escherichia coli N-degron pathway, revealing new sequence-specific contributions from P2-P5 residues.
bioRxiv · May 22, 2025 · PreprintSynthetic Biology