Aditya M. Kunjapur

This study demonstrates that E. coli can efficiently uptake and convert PET deconstruction products into upcycled reduction products with high yields.

This paper demonstrates the use of non-canonical amino acids to enable plant-directed control of bacterial activity in soil.

This paper engineers Escherichia coli to oxidize non-native alcohols using high-expression aryl-alcohol oxidases, enabling air-driven aldehyde synthesis.

This study identifies a nitrogen assimilation bottleneck in Bacillus subtilis growth in plant culture media under shaking conditions.

The paper uses combinatorial mutagenesis to identify stability determinants of the Escherichia coli N-degron pathway, revealing new sequence-specific contributions from P2-P5 residues.

This study investigates how surfactin and glutamate affect the attachment of Bacillus subtilis to tomato roots, showing glutamate promotes colonization while surfactin's role is less significant.

This paper designs an exclusive obligate symbiosis to create a biological containment system where one microbe produces a non-standard amino acid, and another depends on it for survival, demonstrating a chemical-to-biological dependence for biocontainment.

This paper describes a one-pot biocatalytic cascade using PLP-dependent enzymes to synthesize diverse L-phenylalanine derivatives from aldehydes and carboxylic acids under mild conditions.

This paper presents a modular bacterial system for biosynthesizing and incorporating phenylalanine derivatives into proteins using achiral building blocks, reducing reliance on complex chemical synthesis.

The paper develops enzyme cascades for converting lignin-derived carboxylic acids into amines using cell-free and whole-cell biocatalysis, demonstrating new substrates and recyclable systems.

The paper demonstrates that genome engineering of E. coli improves TPAL retention and enables its conversion to valuable products like para-xylylenediamine.

The paper demonstrates that combinatorial gene inactivation of aldehyde dehydrogenases in E. coli reduces aldehyde oxidation in resting cells, improving aldehyde bioconversion.

This paper demonstrates biocatalytic enzyme cascades that convert PET deconstruction products into valuable mono-amine and diamine building blocks.

This paper designs a de novo biosynthetic pathway in E. coli to produce para-nitro-L-phenylalanine (pN-Phe) from glucose, achieving a maximum titer of 819 µM.

This paper demonstrates efficient genetic code expansion in Bacillus subtilis using non-standard amino acids to enable chemical biology tools for studying bacterial biology.

The paper continuously evolves a synthetic auxotrophic E. coli strain and finds it remains contained over 100 days and in co-culture with mammalian cells.

The paper develops a vanillate biosensor for the vanillin pathway in E. coli, identifies improved O-methyltransferases, and demonstrates its utility in metabolic engineering.

The paper shows gene synthesis enables researchers to source genes from more distant organisms in the tree of life, particularly for longer genes.

This paper introduces a method called Post-Translational Proofreading (PTP) to improve the accuracy of detecting non-standard amino acid incorporation into proteins by leveraging the N-end rule pathway.