Design and Evolution of an Orthogonal HaloTag for Multiplexed Labeling in Cells
Benjamin J. Goldberg, Bryan J. Lampkin, Alexander T. Stead, Siena Stanten, Patrick Rabe, Joshua A. Kritzer
This paper designs and evolves an orthogonal HaloTag variant (OrthoTag) that enables multiplexed labeling in cells with high specificity and orthogonality to the original HaloTag.
bioRxiv · Oct 1, 2026 · PreprintBiochemistry