CRISPR interference in a Streptococcus agalactiae Multi-locus Sequence Type 17 Strain
William D. Cutts, Aidan W. Flanagan, Brice Gorman, Audrey Sweten, Bryan Jhonatan Vera Estrada, Vishwas Subash, Benjamin T. Klemp, Kathryn N. Seely, Austin Sandobal, Katelin Stilen, Taksh Vaghela, Areebah Mehvish, Jacob F. Wood, Alexus Govert, Kristin Hobson, , Brandon J. Kim
This study develops a CRISPR interference system in a Streptococcus agalactiae ST-17 strain to enable tunable gene knockdown, demonstrating its utility in studying virulence factors at the blood-brain barrier.
Journal of Bacteriology · Jan 14, 2026Peer-reviewedMicrobiology