CRISPR/Cas9-mediated deletion of a kinetoplast-associated gene attenuates virulence in Leishmania major parasites
Fatemeh Darzi, Ali Khamesipour, Maryam Bahrami, Mahmoud Nateghi-Rostami
This study uses CRISPR/Cas9 to delete the UMSBP gene in Leishmania major, resulting in attenuated parasites with reduced growth and increased apoptosis, which induce a Th1 immune response and partial protection against infection.
bioRxiv · Mar 11, 2024 · PreprintImmunology