Flash properties of Gaussia Luciferase are the result of covalent inhibition after a limited number of cycles
Fenne Marjolein Dijkema, Matilde Knapkøien Nordentoft, Anders Krøll Didriksen, Anders Sværke Corneliussen, Martin Willemoës, Jakob Rahr Winther
The paper investigates the 'flash' kinetics of Gaussia Luciferase, showing that the decay in light signal is due to irreversible enzyme inactivation caused by covalent substrate binding.
Protein Science · Jan 14, 2021Peer-reviewedMolecular Biology