Jakob Rahr Winther

The paper introduces a stable disulfide bond in E. coli OPRT to measure cytosolic redox potential in yeast, finding it to be -300 mV with enhanced stability in glutathione reductase mutants.

The paper develops a yeast-based protein folding sensor to study indel variants, finding that most tolerated indels occur in specific regions and that computational tools can predict their effects.

The paper investigates the 'flash' kinetics of Gaussia Luciferase, showing that the decay in light signal is due to irreversible enzyme inactivation caused by covalent substrate binding.

The paper screens split fluorescent protein variants to identify substitutions that enhance binding affinity.

This study investigates the effects of replacing water with heavy water (D2O) on fission yeast, revealing pleiotropic effects and highlighting the cell integrity pathway's role in D2O tolerance.

This paper introduces a method for high-throughput screening of protein variants using encapsulated E. coli micro-colonies and evaluates biophysical predictors for mutation effects on GFP fluorescence.