Influence of fecal collection conditions and 16S rRNA gene sequencing protocols at two centers on human gut microbiota analysis
Jocelyn Sietsma Penington, Megan A. S. Penno, Katrina Ngui, Nadim J. Ajami, Alexandra J. Roth‐Schulze, Stephen Wilcox, Esther Bandala‐Sanchez, John M. Wentworth, Simon Charles Barry, Cheryl Y. Brown, Jennifer Couper, Joseph F. Petrosino, Anthony T. Papenfuss, Leonard C. Harrison, ENDIA Study Group*, , Peter Vuillermin
This study evaluates how fecal collection conditions and 16S rRNA sequencing protocols affect gut microbiome analysis across two centers, finding that center-specific differences outweigh collection method variations.
Scientific Reports · Mar 6, 2018Peer-reviewedBioinformatics