Frequent sgRNA-barcode Recombination in Single-cell Perturbation Assays
Shiqi Xie, Anne Cooley, Daniel A. Armendariz, Pei Zhou, Gary C. Hon
The paper identifies frequent recombination between sgRNA and barcode in single-cell CRISPR assays, reducing signal-to-noise ratio and suggesting improved methods.
PLoS ONE · Jun 6, 2018Peer-reviewedGenomics