Development of a competition assay to assess the in vitro fitness of dengue virus serotypes using an optimized serotype-specific qRT-PCR
Anne-Fleur Griffon, Loeïza Rault, Clément Tanvet, Etienne Simon‐Lorière, Myrielle Dupont‐Rouzeyrol, Catherine Inizan
This paper develops and validates a multiplex qRT-PCR assay to accurately quantify DENV serotypes in competition assays, revealing DENV-1's enhanced replicative fitness in vitro.
PLoS ONE · Dec 15, 2025Peer-reviewedMicrobiology