Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localization
Saraladevi Muthusamy, Ramesh Raju Vetukuri, Anneli Lundgren, Sungyong Kim, Pruthvi Balachandra Kalyandurg, Åke Strid, Li‐Hua Zhu, Selvaraju Kanagarajan, Peter E. Brodelius
This study produces cyprosin B in Nicotiana benthamiana and shows that removing the PSI domain increases enzymatic activity and alters subcellular localization.
bioRxiv · Aug 27, 2024 · PreprintMolecular Biology