Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localization
Saraladevi Muthusamy, Ramesh Raju Vetukuri, Anneli Lundgren…·Peter E. Brodelius
This study produces cyprosin B in Nicotiana benthamiana and shows that removing the PSI domain increases enzymatic activity and alters subcellular localization.