Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA Polymerase, and a cytosine base editor
Nicole Herrmann May, Anh Cao, Annika Schmid, Fabian Link, Jorge A. Arias-del-Angel, Elisabeth Meiser, Tom Beneke
The paper improves gene editing in Leishmania by combining AsCas12a ultra, T7 RNA Polymerase, and a cytosine base editor, enabling efficient functional screening of genes.
bioRxiv · Feb 28, 2024Molecular Biology