Strategy for unlimited cycles of scarless oligonucleotide directed gene editing in Chlamydomonas reinhardtii
I. L. Ross, S. Budiman, H. P. Le, D. Xiong, F. Hemker, E. A. Millen, M. Oey, B. Hankamer
This paper introduces a scarless gene editing strategy using a cyclical marker system in Chlamydomonas reinhardtii to enable unlimited sequential CRISPR edits.
Plant Physiology · Aug 23, 2024Peer-reviewedPlant Biology