Optimizing expression of Nanobody molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions
M. R. De Groeve, B. J. Laukens, P. G. Schotte
This study improves Nanobody expression in Pichia pastoris by co-expressing four auxiliary proteins under methanol and methanol-free conditions, with HAC1 showing the most significant effect.
bioRxiv · Mar 31, 2023Microbiology