A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader
N. C. Payne, S. Maksoud, B. Tannous, R. Mazitschek
This paper introduces a TR-FRET-based method to quantify protein levels and measure small-molecule target engagement, validated for BRD4 degradation by celastrol.
bioRxiv · Dec 8, 2021Pharmacology & Toxicology