A FRET Ligation Assay using Fluorescent Proteins for Bacterial Sortase Enzymes
Adam Wachsman, Erich G. Walkenhauer, Kaia Stover, Benjamin C Richardson, Sophie N. Jackson, John M. Antos, Jeanine F. Amacher
The paper presents an optimized FRET-based assay using mTurquoise2 and SYFP2 to monitor sortase-mediated ligation products, enabling screening of substrates and sortase variants.
bioRxiv · Aug 24, 2026 · PreprintBiochemistry