Jeanine F. Amacher

This paper investigates how mutations in the β7-β8 loop of Staphylococcus aureus sortase affect catalytic efficiency using NMR and enzyme kinetics.

The paper presents an optimized FRET-based assay using mTurquoise2 and SYFP2 to monitor sortase-mediated ligation products, enabling screening of substrates and sortase variants.

This paper investigates how amino acid variants at position P94 in Staphylococcus aureus sortase A affect substrate binding and enzyme activity, identifying several variants with improved performance.

The paper engineers a peptide that selectively targets a single PDZ domain in a cystic fibrosis model, enhancing chloride efflux.

The paper uses molecular dynamics simulations to model the interaction between Streptococcus pyogenes sortase A and its substrate M protein, revealing stereochemical features and potential transmembrane domain interactions that may enhance catalytic efficiency.

This study characterizes Bacillus anthracis SrtB, showing that residues outside the canonical pentapeptide motif are critical for activity and that engineered variants can improve ligation efficiency.

This study investigates how the WX conserved sequence motif modulates the conformation of the nSrc loop in SH3 domains, affecting ligand specificity.